Group of Nuclear Genomics

Group of Nuclear Genomics

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Faculty staff

Associate Prof. Dr. Kiyotaka Nagaki
E-mail: nagakiATokayama-u.ac.jp
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Lectures: Analytical Molecular Cytogenetics
Keywords: Centromere; Artificial chromosome; Nucleus; Chromatin


Summary of main research topics

Design and synthesis of ‘chromosomes’ carrying new genome information
Nuclei that have very complex structures and various functions are the most important organelles in eukaryotic cells. Nuclear DNA are divided and packed into chromosomes, enabling the accurate transmission of genetic information to daughter cells. Our research group is studying the molecular structures and functions of nuclei and chromosomes, mainly in plants. Our most recent goal is the development of plant artificial chromosomes to elucidate chromosome functional elements: centromeres, telomeres, and replication origins. We are also interested in the relation between chromatin modifications and gene expression.
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Latest publications (for complete and most current publications visit group pages)

(1) Murata, M. Artificial chromosome preparation in arabidopsis. Curr. Protoc. Plant Biol. 1: 53-66. doi: 10.1002/ cppb.20010. (2016. 5.)
(2) Murata, M., Kanatani, A. and Kashihara, K. One-step generation of chromosomal rearrangements in rice. In Chromosome and Genomic Engineering in Plants: Methods and Protocols, Methods in Molecular Biology 1469, Minoru Murata (ed.), Humana Press (Springer Nature), pp. 63-76. doi: 10.1007/978-1-4939-4931-1_5. (2016. 5.)
(3) Nagaki, K. Chromatin immunoprecipitation for detecting epigenetic marks on plant nucleosomes. In Chromosome and Genomic Engineering in Plants: Methods and Protocols, Methods in Molecular Biology 1469, Minoru Murata (ed.), Humana Press (Springer Nature), pp. 197-206. doi: 10.1007/978-1-4939-4931-1_16. (2016. 5.)
(4) Fujimoto, S., Matsunaga, S. and Murata, M. Mapping of T-DNA and Ac/Ds by TAIL-PCR to analyze chromosomal rearrangements. In Chromosome and Genomic Engineering in Plants: Methods and Protocols, Methods in Molecular Biology 1469, Minoru Murata (ed.), Humana Press (Springer Nature), pp. 207-216. doi: 10.1007/ 978-1-4939-4931-1_17. (2016. 5.)