{"id":98,"date":"2021-10-14T01:44:00","date_gmt":"2021-10-13T16:44:00","guid":{"rendered":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/?p=98"},"modified":"2021-10-14T09:52:38","modified_gmt":"2021-10-14T00:52:38","slug":"98","status":"publish","type":"post","link":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/research2.html","title":{"rendered":"\u30a2\u30d6\u30b7\u30b8\u30f3\u9178\u5fdc\u7b54\u6a5f\u69cb\u306e\u5206\u5b50\u907a\u4f1d\u5b66\u7684\u89e3\u6790"},"content":{"rendered":"

\u690d\u7269\u306e\u74b0\u5883\u30b9\u30c8\u30ec\u30b9\u5fdc\u7b54\u6a5f\u69cb\u306e\u7406\u89e3<\/strong>
\n\u690d\u7269\u304c\u74b0\u5883\u306e\u5909\u5316\u3092\u8a8d\u77e5\u3057\u305d\u308c\u306b\u5fdc\u7b54\u3057\u3066\u3044\u308b\u3053\u3068\u306f\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u690d\u7269\u306e\u3069\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u307e\u305f\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5916\u74b0\u5883\u3084\u5185\u74b0\u5883\u306e\u7269\u7406\u7684\u307e\u305f\u306f\u5316\u5b66\u7684\u5909\u5316\u3092\u8a8d\u8b58\u3057\u3001\u3055\u3089\u306b\u305d\u306e\u30b7\u30b0\u30ca\u30eb\u60c5\u5831\u3092\u7d30\u80de\u5185\u307e\u305f\u306f\u500b\u4f53\u5185\u3067\u3069\u306e\u3088\u3046\u306b\u60c5\u5831\u51e6\u7406\u3057\u6700\u9069\u306a\u5fdc\u7b54\u3092\u8d77\u52d5\u3057\u3066\u3044\u308b\u304b\u306f\u3001\u307b\u3068\u3093\u3069\u308f\u304b\u3063\u3066\u3044\u307e\u305b\u3093\u3002\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3053\u306e\u91cd\u8981\u304b\u3064\u9060\u5927\u306a\u554f\u984c\u306b\u7b54\u3048\u308b\u70ba\u306b\u3001\u690d\u7269\u30db\u30eb\u30e2\u30f3\u306e\u3072\u3068\u3064\u3067\u3042\u308b\u30a2\u30d6\u30b7\u30b8\u30f3\u9178\u306b\u6ce8\u76ee\u3057\u3066\u7814\u7a76\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002\u30a2\u30d6\u30b7\u30b8\u30f3\u9178\uff08ABA\uff09\u306f\u3001\u7a2e\u5b50\u306e\u6210\u719f\u306a\u3069\u690d\u7269\u306e\u767a\u751f\u3001\u6210\u9577\u5236\u5fa1\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u6f14\u3058\u3066\u3044\u307e\u3059\u304c\u3001\u4e7e\u71e5\u3001\u5869\u30b9\u30c8\u30ec\u30b9\u5fdc\u7b54\u306b\u304a\u3044\u3066\u3082\u6c17\u5b54\u306e\u958b\u9589\u306e\u5236\u5fa1\u306a\u3069\u4e2d\u5fc3\u7684\u306a\u5f79\u5272\u3092\u62c5\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n

\"\"
\nABA\u306e\u69cb\u9020<\/p>\n

ABA\u5fdc\u7b54\u6a5f\u69cb<\/strong>
\n\u30c8\u30a6\u30e2\u30ed\u30b3\u30b7\u3084\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3092\u4f7f\u3063\u305f\u907a\u4f1d\u5b66\u7684\u7814\u7a76\u3084\u5206\u5b50\u751f\u7269\u5b66\u7684\u7814\u7a76\u306a\u3069\u304b\u3089\u3001ABA\u306e\u4f5c\u7528\u306b\u95a2\u308f\u308b\u591a\u304f\u306e\u306e\u6a5f\u80fd\u5206\u5b50\u304c\u540c\u5b9a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u9006\u907a\u4f1d\u5b66\u7684\u89e3\u6790\u306b\u3088\u308a\u66f4\u306b\u591a\u304f\u306e\u95a2\u9023\u56e0\u5b50\u304c\u5831\u544a\u3055\u308c\u3066\u3044\u307e\u3059(Hirayama & Shinozaki, Trends Plant Sci., 12, 343-, 2007, Plant J., 61, 1041-, 2010)\u3002\u6700\u8fd1\u7d30\u80de\u5185\u3067\u50cd\u304fABA\u53d7\u5bb9\u4f53\u304c\u5831\u544a\u3055\u308c\u307e\u3057\u305f\uff08\u4ee5\u4e0b\u53c2\u7167\uff09\u3002\u3053\u308c\u306b\u3088\u308a\u3001ABA\u5fdc\u7b54\u6a5f\u69cb\u306e\u7406\u89e3\u3082\u52a0\u901f\u3055\u308c\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u7d30\u80de\u30ec\u30d9\u30eb\u3001\u7d44\u7e54\u30ec\u30d9\u30eb\u3067\u306e\u5fdc\u7b54\u6a5f\u69cb\u306e\u7406\u89e3\u306f\u3001\u307e\u3060\u307e\u3060\u591a\u304f\u306e\u672a\u89e3\u660e\u306a\u70b9\u304c\u6b8b\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u4eca\u5f8c\u306f\u3001ABA\u5fdc\u7b54\u6a5f\u69cb\u3068\u4ed6\u306e\u74b0\u5883\u523a\u6fc0\u5fdc\u7b54\u3068\u306e\u30af\u30ed\u30b9\u30c8\u30fc\u30af\u306e\u8a73\u7d30\u306a\u89e3\u6790\u304c\u5fc5\u8981\u3067\u3059\u3002\u79c1\u305f\u3061\u306f\u3001\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u3092\u5bfe\u8c61\u306b\u3057\u3066\u3001\u4e3b\u306b\u907a\u4f1d\u5b66\u7684\u624b\u6cd5\u3092\u7528\u3044\u3066\u65e2\u5b58\u306e\u56e0\u5b50\u306e\u8a73\u7d30\u306a\u6a5f\u80fd\u89e3\u6790\u3084\u3001\u4ed6\u306e\u60c5\u5831\u3068\u306e\u30af\u30ed\u30b9\u30c8\u30fc\u30af\u306b\u304a\u3051\u308b\u5f79\u5272\u306e\u7406\u89e3\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u65b0\u305f\u306aABA\u95a2\u9023\u5909\u7570\u682a\u306e\u63a2\u7d22\u3068\u89e3\u6790<\/strong>
\n\u79c1\u305f\u3061\u306f\u3001\u3044\u304f\u3064\u304b\u306eABA\u9ad8\u611f\u53d7\u6027\u5909\u7570 (ABA hypersensitive germination) ahg1, 2, 3, 11, 12<\/strong><\/em>\u3092\u5206\u96e2\u3057\u89e3\u6790\u3092\u884c\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u3053\u308c\u307e\u3067\u306b\u3059\u3079\u3066\u306e\u539f\u56e0\u907a\u4f1d\u5b50\u3092\u540c\u5b9a\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u3001\u5831\u544a\u3057\u3066\u3044\u307e\u3059(Nishimura et al., Plant J., 44, 972-, 2005, Yoshida et al., Plant Physiol., 140, 2006, 115-, Nishimura et al., Plant J., 50, 935-, 2007, Murayama et al., J. Exp. Bot., 63, 5301-, 2012, Hayashi  & Hirayama, Scientific Reports, 6, 2531, 2016)\u3002<\/p>\n

\u3000\u3000ABA\u9ad8\u611f\u53d7\u6027\u5909\u7570\u682a\u306e\u767a\u82bd\u8a66\u9a13<\/span>
\n\"\"
\nABA\u9ad8\u611f\u53d7\u6027\u5909\u7570\u682a (\u4f8b\uff1bahg3<\/em> )\u306f\u3001ABA\u5b58\u5728\u4e0b\u3067\u767a\u82bd\u53ca\u3073\u751f\u80b2\u304c\u963b\u5bb3\u3055\u308c\u308b\u3002<\/p>\n

\uff0a AHG1, AHG3\u306e\u89e3\u6790<\/strong>
\nahg1-1, ahg3-1<\/em>\u5909\u7570\u306f\u3001\u767a\u82bd\u6642\u306bABA\u9ad8\u611f\u53d7\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u539f\u56e0\u907a\u4f1d\u5b50\u306f\u305d\u308c\u305e\u308c\u7570\u306a\u308b2C\u578b\u8131\u30ea\u30f3\u9178\u5316\u9175\u7d20(PP2C)\u306e\u907a\u4f1d\u5b50\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u308b\u4e8b\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u79c1\u305f\u3061\u304c\u5206\u96e2\u3057\u305f\u5909\u7570\u306f\u3001\u3059\u3079\u3066PP2C\u306e\u6d3b\u6027\u3092\u6d88\u5931\u3055\u305b\u308b\u3082\u306e\u3067\u3057\u305f\u306e\u3067\u3001\u3053\u308c\u3089\u306ePP2C\u306fABA\u5fdc\u7b54\u3092\u6291\u5236\u3059\u308b\u56e0\u5b50\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002\u690d\u7269\u306f\u591a\u304f\u306ePP2C\u3092\u6301\u3064\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\uff08\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306f76\u500b\uff09\u3001\u3053\u306e\u3046\u3061AHG1, AHG3\u3082\u542b\u307e\u308c\u308bcladeA\u3068\u547c\u3070\u308c\u308b\u30b0\u30eb\u30fc\u30d7\u306ePP2C\u304cABA\u5fdc\u7b54\u306b\u95a2\u308f\u3063\u3066\u3044\u308b\u4e8b\u304c\u4eca\u56de\u306e\u7814\u7a76\u304b\u3089\u3082\u660e\u3089\u304b\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306ePP2C\u306f\u5171\u306b\u7a2e\u5b50\u3067\u5f37\u304f\u767a\u73fe\u3057\u3066\u304a\u308a\u307e\u3059\u304c\u3001\u305d\u308c\u305e\u308c\u5171\u901a\u3059\u308b\u6a5f\u80fd\u3068\u5225\u306e\u6a5f\u80fd\u304c\u3042\u308b\u3068\u793a\u5506\u3055\u308c\u308b\u7d50\u679c\u304c\u5f97\u3089\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u3000AHG1, AHG3\u907a\u4f1d\u5b50\u306e\u7a2e\u5b50\u3067\u306e\u767a\u73fe\u306e\u69d8\u5b50 \u3000\u3000\u3000\u3000\u3000ahg1-1ahg3-1<\/em>\u4e8c\u91cd\u5909\u7570\u682a<\/span><\/span>
\n\"1-1\"\u3000\u3000\u3000\u3000\u3000 \"1-3\"<\/p>\n

\u5de6\u56f3: \u305d\u308c\u305e\u308c\u306e\u907a\u4f1d\u5b50\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u5236\u5fa1\u9818\u57df\u306bGUS\u907a\u4f1d\u5b50\uff08\u7523\u7269\u306f\u3042\u308b\u5316\u5b66\u7269\u8cea\u306b\u50cd\u304d\u9752\u8272\u306b\u3059\u308b\uff09\u3092\u9023\u7d50\u3057\u3001\u690d\u7269\u4f53\u306b\u5c0e\u5165\u3002\u9752\u304f\u898b\u3048\u308b\u3068\u3053\u308d\u3067\u907a\u4f1d\u5b50\u304c\u767a\u73fe\u3059\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002AHG1, AHG3\u53cc\u65b9\u3068\u3082\u7a2e\u5b50\u3067\u5f37\u304f\u767a\u73fe\u3059\u308b\u304c\u3001\u767a\u73fe\u3059\u308b\u5834\u6240\u306e\u50be\u5411\u306f\u7570\u306a\u308b\u3002\u53f3\u56f3: ahg1-1ahg3-1\u306e\u4e8c\u91cd\u5909\u7570\u682a\u306eABA\u611f\u53d7\u6027\u3002\u4e8c\u91cd\u5909\u7570\u682a\u3067\u306f\u305d\u308c\u305e\u308c\u306eABA\u9ad8\u611f\u53d7\u6027\u304c\u52a0\u7b97\u3055\u308c\u3066\u3044\u308b\u3088\u3046\u306b\u898b\u3048\u308b\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001AHG1, AHG3\u306e\u6a5f\u80fd\u306f\u5927\u90e8\u5206\u306f\u91cd\u8907\u3057\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u304c\u3001\u305d\u308c\u305e\u308c\u500b\u5225\u306e\u6a5f\u80fd\u3082\u6301\u3064\u3053\u3068\u304c\u308f\u304b\u308b (Nishimura et al., Plant J., 50, 935-, 2007)\u3002<\/p>\n

ABA\u53d7\u5bb9\u4f53<\/strong>
\n\u3042\u308b\u523a\u6fc0\u306e\u60c5\u5831\u4f1d\u9054\u7d4c\u8def\u3092\u7406\u89e3\u3059\u308b\u70ba\u306b\u306f\u3001\u305d\u306e\u523a\u6fc0\u306e\u53d7\u5bb9\u4f53\u3092\u540c\u5b9a\u3059\u308b\u3053\u3068\u304c\u4e0d\u53ef\u6b20\u3067\u3059\u3002ABA\u5fdc\u7b54\u306b\u3064\u3044\u3066\u306f\u3001\u3053\u308c\u307e\u3067\u5e7e\u3064\u304b\u306e\u53d7\u5bb9\u4f53\u5019\u88dc\u304c\u5831\u544a\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u3069\u308c\u3082\u672c\u7269\u3067\u306a\u3044\u3053\u3068\u304c\u305d\u306e\u5f8c\u793a\u3055\u308c\u307e\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u6700\u8fd1\u8907\u6570\u306e\u30b0\u30eb\u30fc\u30d7\u304b\u3089\u7d30\u80de\u8cea\u3067\u50cd\u304fABA\u53d7\u5bb9\u4f53\u304c\u5831\u544a\u3055\u308c\u307e\u3057\u305f(Park et al., Science, 324, 1068- 2009, Ma et al., Science, 324, 1064-, 2009)\u3002\u3053\u308c\u306b\u3088\u308a\u3001ABA\u5fdc\u7b54\u7d4c\u8def\u306e\u89e3\u660e\u304c\u4e00\u6c17\u306b\u9032\u307f\u307e\u3057\u305f\u3002<\/p>\n

\uff0aABA\u60c5\u5831\u4f1d\u9054\u7d4c\u8def\u306e\u89e3\u6790<\/strong>
\n\u3053\u308c\u307e\u3067\u306e\u6211\u3005\u306e\u7814\u7a76\u304b\u3089PP2C\u304cABA\u60c5\u5831\u4f1d\u9054\u306b\u304a\u3044\u3066\u3001\u6291\u5236\u7684\u306b\u50cd\u304f\u91cd\u8981\u306a\u56e0\u5b50\u3067\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002\u4e00\u65b9\u3001SnRK2\u3068\u547c\u3070\u308c\u308b\u4e00\u9023\u306e\u30ea\u30f3\u9178\u5316\u9175\u7d20\u304cABA\u306b\u3088\u308a\u6d3b\u6027\u5316\u3055\u308c\u3001\u307e\u305fABA\u5fdc\u7b54\u3067\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3063\u3066\u3044\u308b\u3053\u3068\u3082\u4ed6\u306e\u7814\u7a76\u30b0\u30eb\u30fc\u30d7\u306e\u89e3\u6790\u308f\u304b\u3063\u3066\u304d\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u3001SnRK2\u306e\u7814\u7a76\u3092\u9032\u3081\u3066\u304d\u305f\u6885\u6fa4\u535a\u58eb\uff08\u8fb2\u5de5\u5927\uff09\u3089\u3068\u5171\u540c\u3067\u3001PP2C\u3068SnRK2\u306e\u95a2\u4fc2\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u7814\u7a76\u306b\u53d6\u308a\u7d44\u307f\u307e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c\u3001PP2C\u3068SnRK2\u306f\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u3001\u7279\u7570\u7684\u306a\u90e8\u4f4d\u306e\u8131\u30ea\u30f3\u9178\u5316PP2C\u304cSnRK2\u306e\u6d3b\u6027\u5316\u3092\u6291\u5236\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u6700\u8fd1\u5831\u544a\u3055\u308c\u305f\u7d30\u80de\u8cea\u306b\u5b58\u5728\u3059\u308bABA\u53d7\u5bb9\u4f53\u3068\u306e\u95a2\u9023\u3082\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f(Umezawa et al., PNAS, 106, 17588-, 2009)\u3002\u3053\u308c\u306b\u3088\u308aABA\u306e\u4e3b\u8981\u306a\u60c5\u5831\u4f1d\u9054\u7d4c\u8def\u3092\u3001\u305d\u306e\u523a\u6fc0\u306e\u53d7\u5bb9\u304b\u3089\u907a\u4f1d\u5b50\u767a\u73fe\u307e\u3067\u4e00\u672c\u306e\u7dda\u3067\u3064\u306a\u3052\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002\u3053\u308c\u307e\u3067\u3001\u8907\u96d1\u3067\u306a\u304b\u306a\u304b\u7406\u89e3\u3067\u304d\u305d\u3046\u306b\u306a\u3044\u3068\u601d\u308f\u308c\u3066\u3044\u305fABA\u60c5\u5831\u4f1d\u9054\u7d4c\u8def\u306e\u4e3b\u8981\u7d4c\u8def\u306f\u3001\u53d7\u5bb9\u4f53\u30fcPP2C\u30fcSnRK2\u30fc\u8ee2\u5199\u56e0\u5b50\u306a\u3069\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u3001\u3067\u69cb\u6210\u3055\u308c\u308b\u30b7\u30f3\u30d7\u30eb\u306a\u69cb\u6210\u3067\u3042\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002<\/p>\n

\u3000\"signal\"
\nPP2C\u306fSnRK2\u3068\u8907\u5408\u4f53\u3092\u5f62\u6210\u3001SnRK2\u306e\u6d3b\u6027\u5316\u3092\u8131\u30ea\u30f3\u9178\u5316\u306b\u3088\u308a\u6291\u5236\u3059\u308b\u3002ABA\u53d7\u5bb9\u4f53(PYR\/RCAR)\u306fABA\u5b58\u5728\u4e0b\u3067PP2C\u3068\u7d50\u5408\u3057\u963b\u5bb3\u3059\u308b\u3002\u3053\u308c\u306b\u3088\u308aSnRK2\u304c\u6d3b\u6027\u5316\u3001\u6a19\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30ea\u30f3\u9178\u5316\u3055\u308c\u305d\u306e\u6a5f\u80fd\u304c\u8abf\u7bc0\u3055\u308c\u308b\u3053\u3068\u3067ABA\u5fdc\u7b54\u304c\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u3002\u30b7\u30ed\u30a4\u30cc\u30ca\u30ba\u30ca\u306b\u306fABA\u53d7\u5bb9\u4f53\u304c14\u3001ABA\u5fdc\u7b54\u306b\u95a2\u308f\u308bPP2C\u3068SnRK2\u304c\u305d\u308c\u305e\u308c9\u30683\u3064\u3042\u308b\u3002\u60c5\u5831\u4f1d\u9054\u7d4c\u8def\u306e\u30b9\u30c6\u30c3\u30d7\u6570\u306f\u5c11\u306a\u304f\u5358\u7d14\u306b\u898b\u3048\u308b\u304c\u3001\u5168\u4f53\u306e\u69cb\u6210\u306f\u3084\u306f\u308a\u8907\u96d1\u3068\u601d\u308f\u308c\u308b(Hirayama & Umezawa, Plant Signaling & Behave., 5, 160-, 2010)\u3002<\/p>\n

\uff0a AHG2\u306e\u89e3\u6790<\/strong>
\nahg2-1<\/em>\u5909\u7570\u682a\u306f\u3001\u767a\u82bd\u6642\u304a\u3088\u3073\u6210\u9577\u3057\u305f\u500b\u4f53\u3067ABA\u9ad8\u611f\u53d7\u6027\u3092\u793a\u3057\u307e\u3059\u3002\u3053\u306e\u5909\u7570\u306e\u539f\u56e0\u907a\u4f1d\u5b50\u306f\u3001poly(A)\u7279\u7570\u7684RNA\u5206\u89e3\u9175\u7d20(PARN)\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u7a81\u304d\u6b62\u3081\u307e\u3057\u305f\u3002PARN\u306a\u3069\u306epoly(A)\u7279\u7570\u7684RNA\u5206\u89e3\u9175\u7d20\u306f\u3001mRNA\u306e\u5206\u89e3\u306e\u521d\u767a\u6bb5\u968e\u3092\u62c5\u3046\u56e0\u5b50\u3067\u3059\u3002\u3053\u306e\u3053\u3068\u304b\u3089\u3001mRNA\u306e\u5206\u89e3\u5236\u5fa1\u304cABA\u5fdc\u7b54\u306b\u5bc6\u63a5\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002ahg2-1<\/em>\u5909\u7570\u682a\u306fABA\u9ad8\u611f\u53d7\u6027\u4ee5\u5916\u306b\u3082ABA\u3068\u62ee\u6297\u7684\u306b\u50cd\u304f\u3068\u8a00\u308f\u308c\u3066\u3044\u308b\u30b5\u30ea\u30c1\u30eb\u9178\u306e\u9ad8\u611f\u53d7\u6027\u3001\u3055\u3089\u306b\u308f\u3044\u6027\u3092\u793a\u3059\u306a\u3069\u3001\u591a\u69d8\u306a\u8868\u73fe\u578b\u304c\u898b\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u3000\u3000ahg2-1<\/span><\/em>\u5909\u7570\u682a\u306fABA\u3068SA\u3092\u591a\u304f\u84c4\u7a4d\u3059\u308b<\/span>
\n\u3000\"ahg2_abasa\"<\/a>\"ahg2_aba\"<\/a><\/p>\n

AHG2\/PARN\u306e\u6a5f\u80fd\u306b\u3064\u3044\u3066\u306e\u30e2\u30c7\u30eb
\n\"\"<\/p>\n

\u7570\u5e38\u306amRNA\u3084\u4e0d\u5fc5\u8981\u306b\u306a\u3063\u305fmRNA\u306f\u901f\u3084\u304b\u306b\u5206\u89e3\u3055\u308c\u308b\u3002\u3053\u306e\u904e\u7a0b\u3067AHG2\/PARN\u304c\u6a5f\u80fd\u3057\u3066\u3044\u308b\u3068\u63a8\u5bdf\u3055\u308c\u308b\u3002\u3053\u306e\u6a5f\u80fd\u304c\u4f4e\u4e0b\u3059\u308b\u3068\u3001\u5206\u89e3\u3055\u308c\u308b\u3079\u304dmRNA\u304c\u84c4\u7a4d\u3057\u3066\u69d8\u3005\u306a\u969c\u5bb3\u304c\u767a\u751f\u3057\u3001\u7d50\u679c\u7684\u306bABA\u9ad8\u611f\u53d7\u6027\u304c\u5f15\u304d\u3053\u3055\u308c\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u3002<\/p>\n

\uff0a AHG2\u306f\u690d\u7269\u7279\u6709\u306e\u6a5f\u80fd\u3092\u6301\u3064<\/strong>
\nahg2-1<\/em>\u3092\u7528\u3044\u305f\u907a\u4f1d\u5b66\u7684\u89e3\u6790\u53ca\u3073\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30fc\u3092\u7528\u3044\u305f\u8a73\u7d30\u306a\u89e3\u6790\u304b\u3089\u3001AHG2\/PARN\u306fpoly(A)\u4ed8\u52a0\u9175\u7d20\u3067\u3042\u308bAGS1\u3068\u5354\u8abf\u3057\u3066\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306emRNA\u306epoly(A)\u9396\u3092\u76f4\u63a5\u8abf\u7bc0\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u308a\u307e\u3057\u305f(Hirayama et al., Nat. Commun., 4, #2247, 2013)\u3002\u4ed6\u306e\u771f\u6838\u751f\u7269\u3067\u306fPARN\u306f\u7d30\u80de\u8cea\u306emRNA\u306epoly(A)\u9664\u53bb\u306b\u95a2\u4e0e\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u5831\u544a\u3055\u308c\u3066\u304a\u308a\u3001\u30aa\u30eb\u30ac\u30cd\u30e9\u3067\u50cd\u304f\u3068\u3044\u3046\u306e\u306f\u3053\u306e\u5831\u544a\u304c\u521d\u3081\u3066\u3067\u3001\u690d\u7269\u306e\u7279\u7570\u6027\u304c\u307e\u305f\u3072\u3068\u3064\u898b\u51fa\u3055\u308c\u307e\u3057\u305f\u3002\u690d\u7269\u306b\u306f\u30b2\u30ce\u30e0\u3092\u4fdd\u6709\u3059\u308b\u30aa\u30eb\u30ac\u30cd\u30e9\u304c\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u3068\u8272\u7d20\u4f53\u3068\u8907\u6570\u3042\u308b\u3053\u3068\u304b\u3089\u3001\u30aa\u30eb\u30ac\u30cd\u30e9\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u5236\u5fa1\u6a5f\u69cb\u304c\u7279\u6b8a\u5316\u3057\u305f\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4eca\u5f8c\u306fAHG2, AGS1\u306e\u8abf\u7bc0\u6a5f\u69cb\u3084\u30b9\u30c8\u30ec\u30b9\u5fdc\u7b54\u4e0b\u3067\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u6a5f\u80fd\u3068\u7d30\u80de\u6a5f\u80fd\u306e\u95a2\u4fc2\u306b\u3064\u3044\u3066\u306e\u89e3\u6790\u304c\u5fc5\u8981\u3067\u3059\u3002<\/p>\n

\u3000AHG2\/PARN\u3068AGS1\/PAP\u306e\u6a5f\u80fd\u30e2\u30c7\u30eb<\/span>
\n\u3000\u3000\"<\/a><\/p>\n

\uff0aAHG1, AHG3\u306f\u7a2e\u5b50\u4f11\u7720\u30fb\u767a\u82bd\u306e\u5236\u5fa1\u3067\u30cf\u30d6\u7684\u5f79\u5272\u3092\u62c5\u3046<\/strong>
\n\u3053\u308c\u307e\u3067\u306e\u89e3\u6790\u304b\u3089AHG1\u3084AHG3\u306f\u4ed6\u306e\u30a2\u30d6\u30b7\u30b7\u30f3\u9178\u5fdc\u7b54PP2C\u3068\u306f\u7570\u306a\u308b\u6a5f\u80fd\u3092\u6301\u3063\u3066\u3044\u308b\u3053\u3068\u304c\u4f3a\u3048\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u3001AHG1\u3068\u76f8\u4e92\u4f5c\u7528\u3059\u308b\u56e0\u5b50\u3092pull-down\u6cd5\u306b\u3088\u308a\u63a2\u7d22\u3057\u307e\u3057\u305f\u3002\u76f8\u4e92\u4f5c\u7528\u56e0\u5b50\u306e\u5019\u88dc\u306b\u306fDOG1\u304c\u898b\u51fa\u3055\u308c\u307e\u3057\u305f\u3002DOG1\u306f\u3001\u7a2e\u5b50\u306e\u4f11\u7720\u5236\u5fa1\u306b\u95a2\u308f\u308b\u56e0\u5b50\u3068\u3057\u3066\u540c\u5b9a\u3055\u308c\u305f\u56e0\u5b50\u3067\u3059\u304c\u3001\u305d\u306e\u751f\u5316\u5b66\u7684\u6a5f\u80fd\u306f\u672a\u89e3\u660e\u3067\u3057\u305f\u3002\u8a73\u7d30\u306a\u7814\u7a76\u304b\u3089AHG1\u3084AHG3\u306f\u3001\u30a2\u30d6\u30b7\u30b7\u30f3\u9178\u306e\u60c5\u5831\u3068DOG1\u304b\u3089\u306e\u4f11\u7720\u306e\u60c5\u5831\u3001\u4e21\u65b9\u3092\u53d7\u3051\u53d6\u308a\u3001\u4e0b\u6d41\u306e\u56e0\u5b50\u306e\u5236\u5fa1\u3092\u884c\u3046\u30cf\u30d6\u7684\u5f79\u5272\u3092\u62c5\u3063\u3066\u3044\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u307e\u3057\u305f\uff08Nishimura et al., Nature Communications, 9, 2132, 2018) \uff08\u4e0b\u56f3\uff09\u3002<\/p>\n

\u3000\u3000\"\"<\/a><\/p>\n

\uff0a \u65b0\u898f\u9244\u5fdc\u7b54\u5236\u5fa1\u56e0\u5b50FEP1\u306e\u540c\u5b9a\u3068\u30a2\u30d6\u30b7\u30b7\u30f3\u9178\u5fdc\u7b54\u3068\u306e\u95a2\u4fc2<\/strong>
\nahg2-1<\/em>\u5909\u7570\u306e\u89e3\u6790\u304b\u3089\u3001\u3053\u306e\u5909\u7570\u682a\u306f\u9244\u304c\u5341\u5206\u306b\u4f9b\u7d66\u3055\u308c\u3066\u3044\u308b\u306b\u3082\u304b\u304b\u308f\u3089\u305a\u6052\u5e38\u7684\u306b\u9244\u6b20\u4e4f\u5fdc\u7b54\u3092\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u7db2\u7f85\u7684\u8ee2\u5199\u7269\u89e3\u6790\u304b\u3089\u3001\u65b0\u898f\u306e\u77ed\u9396\u30da\u30d7\u30c1\u30c9\uff0847aa\uff09\u3092\u30b3\u30fc\u30c9\u3059\u308b\u907a\u4f1d\u5b50FEP1<\/em>\u304c\u3001\u3053\u306e\u5909\u7570\u682a\u3067\u9ad8\u767a\u73fe\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u898b\u51fa\u3057\u307e\u3057\u305f\u3002\u69d8\u3005\u306a\u89e3\u6790\u304b\u3089\u3001FEP1<\/em>\u907a\u4f1d\u5b50\u306f\u9244\u5fdc\u7b54\u6b20\u4e4f\u72b6\u6cc1\u4e0b\u3067\u767a\u73fe\u304c\u8a98\u5c0e\u3055\u308c\u3001FEP1\u306e\u4eba\u70ba\u7684\u8a98\u5c0e\u767a\u73fe\u306e\u307f\u3067\u3001\u690d\u7269\u7d30\u80de\u307e\u305f\u306f\u7d44\u7e54\u5185\u3067\u9244\u306e\u53d6\u308a\u8fbc\u307f\u306b\u95a2\u308f\u308b\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u304c\u8a98\u5c0e\u3055\u308c\u3001\u307e\u305f\u9244\u304c\u84c4\u7a4d\u3055\u308c\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u9244\u5fdc\u7b54\u907a\u4f1d\u5b50\u767a\u73fe\u8a98\u5c0e\u306f\u3001\u3053\u308c\u307e\u3067\u306b\u77e5\u3089\u308c\u3066\u3044\u306a\u3044\u6a5f\u69cb\u306b\u3088\u308b\u3053\u3068\u3082\u308f\u304b\u308a\u307e\u3057\u305f\u3002CRISPR\/Cas9\u306b\u3088\u308bfep1<\/em>\u6b20\u640d\u5909\u7570\u306e\u4f5c\u6210\u3068\u305d\u306e\u89e3\u6790\u304b\u3089\u3001FEP1\u306f\u500b\u4f53\u5168\u4f53\u306e\u9244\u6b20\u4e4f\u5fdc\u7b54\u7d71\u5fa1\u306b\u95a2\u308f\u308b\u3053\u3068\u304c\u793a\u5506\u3055\u308c\u307e\u3057\u305f(Hirayama et al., Plant Cell Physiol., 2018) (\u4e0b\u56f3\uff09\u3002\u73fe\u5728\u3001\u30a2\u30d6\u30b7\u30b7\u30f3\u9178\u5fdc\u7b54\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u6a5f\u80fd\u3068\u306e\u95a2\u9023\u3092\u8996\u91ce\u306b\u5165\u308c\u306a\u304c\u3089\u3001FEP1\u304c\u95a2\u308f\u308b\u5236\u5fa1\u6a5f\u69cb\u306e\u5206\u5b50\u30ec\u30d9\u30eb\u3067\u306e\u89e3\u660e\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n

     \"\"<\/a><\/p>\n

>>> \u305d\u306e\u4ed6\u306e\u5909\u7570\u682a<\/a>
\n>>> \u30c7\u30fc\u30bf\u99c6\u52d5\u578b\u4f5c\u7269\u30c7\u30b6\u30a4\u30f3\u6280\u8853\u306e\u958b\u767a<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u690d\u7269\u306e\u74b0\u5883\u30b9\u30c8\u30ec\u30b9\u5fdc\u7b54\u6a5f\u69cb\u306e\u7406\u89e3 \u690d\u7269\u304c\u74b0\u5883\u306e\u5909\u5316\u3092\u8a8d\u77e5\u3057\u305d\u308c\u306b\u5fdc\u7b54\u3057\u3066\u3044\u308b\u3053\u3068\u306f\u3088\u304f\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u690d\u7269\u306e\u3069\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u307e\u305f\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5916\u74b0\u5883\u3084\u5185\u74b0\u5883\u306e\u7269\u7406\u7684\u307e\u305f\u306f\u5316\u5b66\u7684\u5909\u5316\u3092\u8a8d\u8b58\u3057\u3001\u3055\u3089\u306b\u305d\u306e\u30b7\u30b0\u30ca\u30eb\u60c5\u5831\u3092\u7d30\u80de … \u7d9a\u304d\u3092\u8aad\u3080 →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[6],"tags":[],"class_list":["post-98","post","type-post","status-publish","format-standard","hentry","category-reserch"],"_links":{"self":[{"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/posts\/98","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/comments?post=98"}],"version-history":[{"count":25,"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/posts\/98\/revisions"}],"predecessor-version":[{"id":1188,"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/posts\/98\/revisions\/1188"}],"wp:attachment":[{"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/media?parent=98"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/categories?post=98"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.rib.okayama-u.ac.jp\/ers\/wp-json\/wp\/v2\/tags?post=98"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}