{"id":82,"date":"2021-03-24T10:40:20","date_gmt":"2021-03-24T01:40:20","guid":{"rendered":"https:\/\/www.rib.okayama-u.ac.jp\/english\/?page_id=82"},"modified":"2021-03-24T10:40:25","modified_gmt":"2021-03-24T01:40:25","slug":"biolistic-particle-delivery-system","status":"publish","type":"page","link":"https:\/\/www.rib.okayama-u.ac.jp\/english\/collaboration\/biolistic-particle-delivery-system\/","title":{"rendered":"Biolistic particle delivery system"},"content":{"rendered":"<p><strong>Models\uff1aPDS-1000\/He \uff08Bio-Rad\uff09<\/strong><\/p>\n<p>The PDS-1000\/He system accelerates nucleic acid\u2013coated gold or tungsten microparticles (0.6\u20131.6 \u00b5m) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.<br \/>The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they travel toward the target cells<\/p>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Models\uff1aPDS-1000\/He \uff08Bio-Rad\uff09 The PDS-1000\/He system accelerates nucleic acid\u2013coated gold or tungsten microparticles (0.6\u20131.6 \u00b5m) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. To increase the efficiency of the process, the chamber may be evacuated to subatmospheric pressures, reducing the frictional drag on the microparticles as they [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":27,"menu_order":51,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-82","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.6 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Biolistic particle delivery system - Institute of Plant Science and Resources<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.rib.okayama-u.ac.jp\/english\/collaboration\/biolistic-particle-delivery-system\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Biolistic particle delivery system - Institute of Plant Science and Resources\" \/>\n<meta property=\"og:description\" content=\"Models\uff1aPDS-1000\/He \uff08Bio-Rad\uff09 The PDS-1000\/He system accelerates nucleic acid\u2013coated gold or tungsten microparticles (0.6\u20131.6 \u00b5m) to velocities necessary to transfect cells, tissues, or organelles. The system uses a burst of high-pressure helium gas to accelerate a plastic macrocarrier disk carrying microparticles toward target cells.The helium pressure used to propel the macrocarrier is determined by the choice of rupture disk, a plastic seal designed to burst at a specific pressure. A stopping screen retains the macrocarrier while allowing the microparticles to pass through and penetrate the target cells. 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