植物成長制御グループでは環境ストレス下での植物の成長制御機構を研究してます。

新着情報

2016年3月30日

ALMTのキメラタンパク質を植物細胞に発現させることで３価金属イオンへの応答性が高まることを明らかにした論文がBBA−Biomembranesに掲載されされました。

2016年5月16日

ミヤコグサALMTが根粒近くで発現し、リンゴ酸だけでなくフマル酸やコハク酸も輸送可能なジカルボン酸トランスポーターであることを明らかにしました。京都大学・生存研との共同研究です。

2016年9月20-22日　　　

日本土壌肥料学会（佐賀大学）で2演題発表しました

2016年9月11日

トマトの果実成熟の過程で発現する２つのSlALMT4,SlALMT5を同定しました。２つは果実の種子につながる維管束で発現しており、種子へのリンゴ酸輸送が推測されました。そこで、SlALMT5の過剰発現トマトを作出し、解析した結果、種子中のリンゴ酸とクエン酸含量が増大していたことから、SlALMT5は種子の有機酸含量の調節に関わることが解かりました。　この研究は岡山大学・植物研と農学部の共同研究です。論文はPlant Cell Physiol. 11月に掲載されました。

2017年3月5-7日

カナダ・バンクーバーで開催された12th Keele Meeting on Aluminiumに参加し、発表してきました（山本、佐々木、苅谷）

2017年3月16-18日

日本植物生理学会（鹿児島）にて発表してきました（佐々木、苅谷）

2017年3月

苅谷くんの論文がSSPN誌に掲載されました

Publications

Takanashi, K., Yamada, Y., Sasaki, T., Yamamoto, Y., Sato, F., Yazaki, K. (2017) A multidrug and toxic compound extrusion transporter mediates berberine accumulation into vacuoles in Coptis japonica. Phytochemistry (in press)

Kariya, k., Sameeullah, M., Sasaki, T., Yamamoto, Y. (2017) Overexpression of the sucrose transporter NtSUT1 alleviates aluminum-incuced inhibition of root elongation in tobacco (Nicotiana tabacum L.). Soil Sci. Plant Nutri. 63, 45-54.

Sasaki, T., Tsuchiya, Y., Ariyoshi, M., Nakano, R., Ushijima, K., Kubo, Y., Mori, I.C., Higashiizumi, E., Galis, I., Yamamoto, Y. (2016) Two members of the aluminum-activated malate transporter family, SlALMT4 and SlALMT5, are expressed during fruit development and the overexpression of SlALMT5 alters organic acid contents in seeds in tomato (Solanum lycopersicum). Plant Cell Physiol. 57, 2367-2379.

Takanashi, K., Sasaki, T., Kan, T., Saida, Y., Sugiyama, A., Yamamoto, Y., Yazaki, K. (2016) A dicarboxylate transporter, LjALMT4, mainly expressed in nodules of Lotus japonicus. Mol Plant Microbe Interact. 29, 584-592.

Sasaki, T., Tsuchiya, Y., Ariyoshi, M., Ryan, P.R., Yamamoto, Y. (2016) A chimeric protein of aluminum-activated malate transporter generated from wheat and Arabidopsis shows enhanced response to trivalent cations. Biochim. Biophys. Acta 1858, 1427-1435

Sasaki, T., Tsuchiya, Y., Ariyoshi, M., Ryan, P.R., Furuichi, T., Yamamoto, Y. (2014) A domain-based approach for analyzing the function of aluminum-activated malate transporters from wheat (Triticum aestivum) and Arabidopsis thaliana in Xenopus oocytes. Plant Cell Physiol. 55, 2126-2138.

Yamashita, J., Enomoto, T., Yamada, M., Ono, T., Hanafusa, T., Nagamatsu, T., Sonoda, S., Yamamoto Y. (2014) Estimation of soil-to-plant transfer factors of radiocesium in 99 wild plant species grown in arable lands 1 year after the Fukushima 1 Nuclear Power Plant accident. J. Plant Res. 127, 11-22.

Sameeullah, M., Sasaki, T, Yamamoto, Y. (2013) Sucrose transporter NtSUT1 confers aluminum tolernace on cultured cells of tobacco (Nicotiana tabacum L.). Soil. Sci. Plant Nutri. 59, 756-770.

Kariya, K., Demiral,T., Sasaki, T., Tsuchiya,T., Turkan, I., Sano, T., Hasezawa, S., Yamamoto, Y. (2013) A novel mechanism of aluminium-induced cell death involving vacuolar processing enzyme and vacuolar collapse in tobacco cell line BY-2. J. Inorg. Biochem. 128, 196-201.

Wu, D., Zhao, M., Shen, S., Fu, Y., Sasaki, T., Yamamoto, Y., Wei ,W., Shen, H. (2013) Al-induced secretion of organic acid, gene expression and root elongation in soybean roots. Acta Physiol. Plant. 35:223-232.

Chan, T., Shimizu, Y., Pospi?il, P., Nijo, N., Fujiwara, A., Taninaka, Y., Ishikawa, T., Hori, H., Nanba, D., Imai, A., Morita, N., Yoshioka-Nishimura, M., Izumi, Y., Yamamoto, Y., Kobayashi, H., Mizusawa, N., Wada, H., Yamamoto, Y. (2012) Quality control of photosystem II: Lipid peroxidation accelerates photoinhibition under excessive illumination. PLoS ONE 7;e52100

Hoque, T.S,, Okuma, E., Uraji, M., Furuichi, T., Sasaki, T., Hoque, M.A., Nakamura, Y., Murata, Y. 2012 Inhibitory effects of methylglyoxal on light-induced stomatal opening and inward K+ channel activity in Arabidopsis. Biosci. Biotechnol. Biochem. 76: 617-619.

Ryan, P.R. Tyerman, S.D., S. Sasaki, T.,Furuichi, T., Yamamoto, Y., Zhang, W.-H., Delhaize, E. (2011) The identification of aluminium-resistance genes provides opportunities for enhancing crop production on acid soils. J. Exp. Bot. 62, 9-20.

Ryan, P.R. Raman, H. Gupta, S. Sasaki, T., Yamamoto, Y., Delhaize, E. (2010) Multiple origins of aluminium resistance in hexaploid wheat are derived from Aegilops tauschii and from more recent cis mutations of TaALMT1. Plant J. 64, 446-455.

Furuichi, T., Sasaki, T., Tsuchiya, Y., Ryan, P.R., Delhaize, E., Yamamoto, Y. (2010) An extracellular hydrophilic carboxyl-terminal domain regulates the activity of TaALMT1, the aluminum-activated malate transport protein of wheat. Plant J. 64, 47-55.

Sasaki, T., Mori, I.C., Furuichi, T., Munemasa, S., Toyooka, K., Matsuoka, K., Murata, Y., Yamamoto, Y. (2010) Closing plant stomata requires a homolog of an aluminum-activated malate transporter. Plant Cell Physiol. 51, 354-365.

Abdel-Basset, R., Ozuka, S., Demiral, T., Furuichi, T., Sawatani, I., Baskin, T.I., Matsumoto, H., Yamamoto, Y. (2010) Aluminum reduces sugar uptake in tobacco cell cultures: A potential cause of inhibited elongation but not of toxicity. J. Exp. Bot. 61, 1597-1610.

Kim, Y.-S., Park, W., Nian, H., Sasaki, T., Ezaki, B., Jang, Y.-S., Chung, G.-C., Bae, H.-J., Ahn, S.-J. (2010) Aluminum tolerance associated with enhancement of plasma membrane H+-ATPase in the root apex of soybean. Soil Sci. Plant Nutri. 56, 140-149.

Tani, A., Kawahara, T., Yamamoto, Y., Kimbara, K. and Kawai, F. (2010) Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis. J. Biosci. Bioeng. 109:453-458.

Yin, L., Wang, S., Eltayeb, A.E., Uddin, Md. I. , Yamamoto, Y., Tsuji, W., Takeuchi, Y. and Tanaka, K. (2010) Overexpression of dehydroascorbate reductase, but not monodehydroascorbate reductase, confers tolerance to aluminum stress in transgenic tobacco. Planta 231: 609-621.

Raman, H., Ryan, P.R., Raman, R., Stodart, B.J., Zhang, K., Martin, P., Wood, R., Sasaki, T., Yamamoto, Y., Mackay, M., Hebb, D.M., Delhaize, E. (2008) Analysis of TaALMT1 traces the transmission of aluminum resistance in cultivated common wheat (Triticum aestivum L.). Theor. Appl. Genet. 116, 343-354.

Zhang, W.-H., Ryan, P.R., Sasaki, T., Yamamoto, Y., Sullivan, W., Tyerman, S.D. (2008) Characterisation of the TaALMT1 protein as an Al3+-activated anion channel in transformed tobacco (Nicotiana tabacum L.) cells. Plant Cell Physiol. 49, 1316-1330.

Panda, S.K., Yamamoto, Y., Kondo, H., and Matsumoto, H. (2008) Mitochondrial alterations related to programmed cell death in tobacco cells under aluminium stress. C. R. Biologies 331: 597-610.

Tani, A., Inoue, C., Tanaka, Y., Yamamoto, Y., Kondo, H., Hiradate, S., Kimbara, K., Kawai, F. (2008) The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis. Microbiology 154, 3437-37446.

Raman, H., Ryan, P., Raman, R., Stodart, B., Zhang, K., Martin, P., Wood, R., Sasaki, T., Yamamoto, Y., Mackay, M., Hebb, D., Delhaize, E. (2008) Analysis of TaALMT1 traces the transmission of aluminum resistance in cultivated common wheat (Triticum aestivum L.). Theor. Appl. Genet. 116, 343-354.

Motoda, H., Sasaki, T., Kano, Y., Ryan, P.R., Delhaize, E.., Matsumoto, H., and Yamamoto, Y. (2007) The membrane topology of ALMT1, an aluminum-activated malate transport protein in wheat (Triticum aestivum). Plant Signal. Behav. 2, 467-472.

Kikui, S., Sasaki, T., Osawa, H., Matsumoto, H., and Yamamoto, Y. (2007) Malate enhances recovery from Al-caused inhibition of root elongation in wheat. Plant Soil 290, 1-15.

Sasaki, T., Ryan, P.R., Delhaize, E., Hebb, D.M., Ogihara, Y., Kawaura, K., Noda, K., Kojima, T., Toyoda, A., Matsumoto, H., Yamamoto, Y. (2006) Sequence upstream of the wheat (Triticum aestivum L.) ALMT1 gene and its relationship to aluminum resistance. Plant Cell Physiol. 47, 1343-1354.

Hoekenga, O. A., Maron, L., G., Pineros, M., A., Cancado, G., M., Shaff, J., Kobayashi, Y., Ryan, P. R., Dong, B., Delhaize, E., Sasaki, T., Matsumoto, H., Yamamoto, Y., Koyama, H., Kochian L.V. (2006) AtALMT1, which encodes a malate transporter, is identified as one of several genes critical for aluminum tolerance in Arabidopsis. Proc. Natl. Acad. Sci. USA 103, 9738-9743.

Shen, H., Chen, J., Wang, Z., Yang, C., Sasaki, T., Yamamoto, Y., Matsumoto, H. and Yan, X. 2006. Root plasma membrane H+-ATPase is involved in the adaptation of soybean to phosphorus starvation. Journal of Experimental Botany 57:1353-1362.

Kikui, S., Sasaki, T., Maekawa M., Miyao, M., Hirochika, H., Matsumoto, H and Yamamoto, Y. (2005) Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination. J. Inorg. Biochem. 99, 1837-1844.

Lin C., Yu, Y., Kadota, T., Iwata, M., Umemura, K., Furuichi. T., Kuse, M., Isobe, M., Yamamoto, Y., Matsumoto, H., Yoshizuka, K. and Kawano, T. (2005) Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells. Biochem. Biophys. Res. Commun. 332, 823-830.

Raman, H., Zhang, K., Cakir M., Appels, R., Garvin, D.F., Maron, L.G., Kochian, L.V., Moroni, J.S., Raman, R., Imtiaz, M., Drake-Brockman, F., Waters, I., Martin, P., Sasaki, T., Yamamoto, Y., Matsumoto, H., Hebb, D.M. Delhaize E., and Ryan, P.R. (2005) Molecular characterization and mapping of ALMT1, the aluminum-tolerance gene of bread wheat (Triticum aestinvum L.). Genome 48, 781-791.

Shen, H., He, L. F., Sasaki, T., Yamamoto, Y., Zheng, S., J., Ligaba, A., Yan, X. L., Ahn, S., J., Yamaguchi, M., Sasakawa, H. and Matsumoto, H. (2005) Citrate secretion coupled with the modification of soybean root tip under aluminum stress. Up-regulation of transcription, translation, and threonine-oriented phosphorylation of plasma membrane H+-ATPase. Plant Physiol. 138, 287-296.

Yamaguchi, M., Sasaki, T., Sivaguru, M., Yamamoto, Y., Osawa, H., Ahn, S. J. and Matsumoto, H. (2005) Evidence for the plasma membrane localization of Al-activated malate transporter (ALMT1). Plant Cell Physiol. 46, 812-816.

Sivaguru, M., Yamamoto, Y., Rengel, Z., Ahn, S. J. and Matsumoto, H. (2004) Early events responsible for aluminum toxicity symptoms in suspension-cultured tobacco cells. New Phytol. 165, 99-109.

Delhaize, E., Ryan, P.R., Hebb, D.M, Yamamoto, Y., Sasaki, T., Matsumoto, H. (2004) Engineering high-level aluminum tolerance in barley with the ALMT1 gene. Proc. Natl. Acad. Sci. USA 42, 15249-15254.

Kobayashi, Y., Yamamoto, Y., Matsumoto, H. (2004) Studies on the mechanism of aluminum tolerance in pea (Pisum sativaum L.) using aluminum-tolerant cultivar ‘Alaska’ and aluminum-senseitive cultivar ‘Hyogo’. Soil Sci. Plant Nutri. 50, 197-204.

Sasaki, T., Yamamoto, Y., Ezaki, B., Katsuhara, M., Ahn, S.J., Ryan, P.R., Delhaize, E. and Matsumoto, H. 2004. A wheat gene encoding an aluminium-activated malate transporter. Plant J. 37, 645-653.

佐々木孝行・山本洋子「アルミニウム耐性の分子機構：有機酸トランスポーターによる制御」蛋白質核酸酵素　2007年5月号増刊　植物における環境と生物ストレスに対する応答　共立出版 Vol.52, 619-624 (2007)

佐々木孝行・山本洋子「アルミニウム毒性の多様性と作物にみられる耐性の分子メカニズム」化学と生物 Vol.43,569-578 (2005)


2004年以降、主な業績です。その他の業績については、メンバーのページ（準備中）からごらん下さい

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