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Genome analysis

The project ‘Identification of genes of important traits and their application in barley breeding’ has just finished with support of Bio-oriented Technology Research Advancement Institution (BRAIN). The project aimed to sequence genes on chromosome 3H and isolate genes responsible for brewing traits and stress tolerances. The full length cDNA projects on barley are also conducted by National Bioresource Project and Genome diversity analysis project by MAFF. Please visit Databases page for sequence data.

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Sequencing Currently, barley genome is sequenced by the international barley sequencing consortium using next generation sequencers.
cDNA collections and applications 135,000ESTs and 5,000 FLcDNAs have been sequenced and published at databases. Of these, 80,000 clones were distributed in NBRP project. GeneChip Barley 1 Array was developed from EST sequences.
BAC clones A BAC library by Japanese cultivar was developed and distributed. Membrane screening system (16 large filters) and DNA plate pools for PCR screening are also distributed.
EST maps A high density transcript map was developed and published as an electornic map (cMAP).
Comparative analysis A diploid wheat genetic map have been published in paper and database.

Genome analysis