Publication (after 2016)
Research papers
● 2025
Hisano H, Sakai H, Hamaoka M, Munemori H, Abe F, Meints B, Sato K, Hayes PM (2025) Rapid development of naked malting barley germplasm through targeted mutagenesis. Molecular Breeding 45:32 https://doi.org/10.1007/s11032-025-01553-5
Mahadevan, N., Fernanda, R., Kouzai, Y., Kohno, N., Nagao, R., Nyein, K. T., Watanabe, M., Sakata, N., Matsui, H., Toyoda, K., Ichinose, Y., Mochida, K., Hisano, H., & Noutoshi, Y. (2025). Distinct Infection Mechanisms of Rhizoctonia solani AG-1 IA and AG-4 HG-I+II in Brachypodium distachyon and Barley. Life, 15(2), 235. https://doi.org/10.3390/life15020235
● 2024
Matsushima, R., Hisano, H., Kim, JS. McNelly R, Oitome NF, Seung D, Fujita N, Sato K. 2024. Mutations in starch BRANCHING ENZYME 2a suppress the traits caused by the loss of ISOAMYLASE1 in barley. Theor Appl Genet 137, 212. https://doi.org/10.1007/s00122-024-04725-7
Kishi-Kaboshi, M., Abe, F., Chono, M., Yamaji, N., & Sato, K. (2024). Evaluation of grain dormancy under field conditions in a wheat (Triticum aestivum) qsd1 triple mutant. Crop Science, 1–11. https://doi.org/10.1002/csc2.21403
● 2023
Kishi-Kaboshi, M., Abe, F., Kamiya, Y., Kawaura, K., Hisano, H. and Sato, K. Optimizing genome editing efficiency in wheat: Effects of heat treatments and different promoters for single guide RNA expression. Plant Biotechnology 40, 237-245. (2023) doi.org/10.5511/plantbiotechnology.23.0717a
Matsushima, R., Hisano, H., Galis, I., Miura, S., Crofts, N., Takenaka, Y., Oitome, N.F., Ishimizu, T., Fujita, N. and Sato, K. FLOURY ENDOSPERM 6 mutations enhance the sugary phenotype caused by the loss of ISOAMYLASE1 in barley. Theoretical and Applied Genetics 136, 94. (2023) doi.org/10.1007/s00122-023-04339-5
Koide H, Hisano H, Yaeno T. 2023. CRISPR/Cas9-based generation of mlo mutants for allelic complementation experiments to elucidate MLO function in barley. Journal of General Plant Pathology. 89, 153–158 (2023) doi.org/10.1007/s10327-023-01120-w
Kondo, H.; Sugahara, H.; Fujita, M.; Hyodo, K.; Andika, I.B.; Hisano, H.; Suzuki, N. Discovery and Genome Characterization of a Closterovirus from Wheat Plants with Yellowing Leaf Symptoms in Japan. Pathogens 2023, 12, 358. doi.org/10.3390/pathogens12030358
● 2022
Yoshikawa T, Hisano H, Hibara K-I, Nie J, Tanaka Y, Itoh J-I, Taketa S. 2022. A bifurcated palea mutant infers functional differentiation of WOX3 genes in flower and leaf morphogenesis of barley. AoB PLANTS 14: plac019. doi.org/10.1093/aobpla/plac019
Gu M, Huang H, Hisano H, Ding G, Huang S, Mitani-Ueno N, Yokosho K, Sato K, Yamaji N, Ma JF. 2022. A crucial role for a node-localized transporter, HvSPDT, in loading phosphorus into barley grains. New Phytologist 234: 1249-1261. doi.org/10.1111/nph.18057
Hisano, H., Hoffie, R.E., Abe, F., Munemori, H., Matsuura, T., Endo, M., Mikami, M., Nakamura, S., Kumlehn, J. and Sato, K. (2022) Regulation of germination by targeted mutagenesis of grain dormancy genes in barley. Plant Biotechnology Journal 20, 37-46.
● 2021
Yaeno, T., Wahara, M., Nagano, M., Wanezaki, H., Toda, H., Inoue, H., Eishima, A., Nishiguchi, M., Hisano, H., Kobayashi, K., Sato, K., Yamaoka, N. 2021. RACE1, a Japanese Blumeria graminis f. sp. hordei isolate, is capable of overcoming the mlo-mediated penetration resistance in barley. PLOS ONE https://doi.org/10.1371/journal.pone.0256574
Liu, Y., Luo, W., Linghu, Q., Abe, F., Hisano, H., Sato, K., Kamiya, Y., Kawamura, K., Onishi, K., Endo, M., Toki, S., Hamada, H., Nagira, Y., Taoka, N., Imai, R. 2021. In planta genome editing in commercial wheat varieties. Frontiers Plant Sci. 10.3389/fpls.2021.648841.
● 2020
Lei, G. J. Fujii-Kashino, M., Wu, D. Z., Hisano, H., Saisho, D., Deng, F., Yamaji, N., Sato, K., Zhao, F., Ma, J. F. 2020. Breeding low cadmium barley through introgression of a Sukkula-like transposable element for human health. Nat. Food 1:489–499.
Nagai, K., Mori, Y., Ishikawa, S., Furuta, T., Gamuyao, R., Niimi, Y., Hobo, T., Fukuda, M., Kojima, M., Takebayashi, Y., Fukushima, A., Himuro, Y., Kobayashi, M., Ackley, W., Hisano, H., Sato, K., Yoshida, A., Wu, J., Sakakibara, H., Sato, Y., Tsuji, H., Akagi, T. and Ashikari, M. 2020. Antagonistic regulation of gibberellin response during growth of rice stem. Nature 10.1038/s41586-020-2501-8
Abe, F., Ishida, Y., Hisano, H., Endo, M., Komari, T., Toki, S., Sato, K. 2020. Using Genome Editing to Produce Multiple Mutants in Wheat. StarProtocols 1, 100053. https://doi.org/10.1016/j.xpro.2020.100053
● 2019
Abe, F., Haque, E., Hisano, H., Tanaka, T., Kamiya, Y., Mikami, M., Kawaura, K., Endo, M., Onishi, K., Hayashi, T. and Sato, K. 2019. Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat. Cell Rep. 28: 1362-1369.
Wu, Z., Wang, N., Hisano, H., Cao, Y., Wu, F., Liu, W., Bao, Y., Wang Z.-Y., and Fu, C. 2019. Simultaneous regulation of F5H in COMT-RNAi transgenic switchgrass alters effects of COMT suppression on syringyl lignin biosynthesis. Plant Biotechnol. J. 17: 836-845.
Matsushima R, Hisano H. 2019. Imaging Amyloplasts in the Developing Endosperm of Barley and Rice. Scientific Reports 9: 3745.
● 2018
Kodama, A., Narita, R., Yamaguchi, M., Hisano, H., Adachi, S., Takagi, H., Ookawa, T., Sato, K. and Hirasawa, T. 2018. Genotypic Difference in Grain Fertility under Salt Stress and the Responsible Quantitative Trait Loci in Barley. Breed. Sci. 68: 561-570.
Fujii-Kashino, M., Yamaji, N., Yamane, M., Saisho, D., Sato, K. and Ma, J. F. 2018. Both retrotransposon insertion and demethylation regulate aluminum tolerance of European barley for postdomestication expansion to acid soil. Plant Physiol. 178:716-727.
Mashiguchi, K., Hisano, H., Takeda-Kamiya, N., Takebayashi, Y., Ariizumi, T., Gao, Y., Ezura, H., Sato, K., Zhao, Y., Hayashi, K. and Kasahara, H. 2018. Agrobacterium tumefaciens enhances biosynthesis of two distinct auxins in the formation of crown galls. Plant Cell Physiol. 60:29-37.
Kazuhiro Sato, Hiroshi Hisano, Satoko Matsumoto, Tian-Su Zhou and Makoto Kihara. 2018. Detection of QTLs controlling alpha-amylase activity in a diversity panel of 343 barley accessions. Mol. Breed. 38:14.
● 2017
Gupta, A., Hisano, H., Hojo, Y., Matsuura, T., Ikeda, Y., Mori, I.C., Senthil-Kumar, M. Global profiling of phytohormone dynamics during combined drought and pathogen stress in Arabidopsis thaliana reveals ABA and JA as major regulators. Scientific Reports 7: 4017.
Hiroshi Hisano, Brigid Meints, Matthew J. Moscou, Luis Cistue, Begoña Echávarri, Kazuhiro Sato and Patrick M. Hayes. 2017. Selection of transformation efficient lines in the Oregon Promise barley mapping population based on TFA (transformation amenability) haplotype. Plant Cell Reports 36: 611-620.
Hiroshi Hisano, Kazuki Sakamoto, Hiroki Takagi, Ryohei Terauchi, and Kazuhiro Sato. 2017. Exome QTL-seq maps monogenic locus and QTLs in barley. BMC Genomics 18:125.
● 2016
Hisano Hisano and Kazuhiro Sato. 2016. Genomic regions responsible for amenability to Agrobacterium-mediated transformation in barley. Scientific Reports 6: 37505.
Analiza Grubanzo Tagle, Izumi Chuma, Hiroshi Hisano, Kazuhiro Sato and Yukio Tosa. 2016. Genetic analysis of the resistance of barley to cryptic species of Pyricularia. Journal of General Plant Pathology 82:302–306.
Hiroshi Hisano, Mai Tsujimura, Hideya Yoshida, Toru Terachi and Kazuhiro Sato. 2016. Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare). BMC Genomics 17:824.
Hiroshi Hisano, Takakazu Matsuura, Izumi C. Mori, Miki Yamane and Kazuhiro Sato. 2016. Endogenous hormone levels affect the regeneration ability of callus derived from different organs in barley. Plant Physiol. Biochem. 99: 66–72.
Takahagi, K. , Uehara-Yamaguchi, Y., Yoshida, T., Sakurai, T., Shinozaki K., Mochdia, K. & Saisho, D. 2016. Analysis of single nucleotide polymorphisms based on RNA sequencing data of diverse bio-geographical accessions in barley. Scientific Reports 6 : 33199.
Saisho, D., Takumi, S. & Matsuoka, Y. 2016. Salt tolerance during germination and seedling growth of wild wheat Aegilops tauschii and its impact on the species range expansion. Scientific Reports 6: 38554.
Book chapter (including Japanese)
Hisano H, Hoffie RE, Kumlehn J, Sato K. (2024) Targeted Modification of Grain Dormancy Genes in Barley. In Seed Dormancy: Methods and Protocols, Ed. by N. Kawakami and K. Sato. Springer Nature. pp. 149–161 doi.org/10.1007/978-1-0716-3965-8_14
Abe F, Kamiya Y, Ishida Y, Hisano H, Kawaura K, Komari T., Sato K. (2024) Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat. In Seed Dormancy: Methods and Protocols, Ed. by N. Kawakami and K. Sato. Springer Nature. pp. 137-148 doi.org/10.1007/978-1-0716-3965-8_13
佐藤和広・草場信・石井孝佳・中園幹生・久野裕・門田有希 植物の遺伝と育種（第3版）（佐藤和広・草場信・中園幹生 編）. 朝倉書店. ISBN-13: 9784254420470 (2023.4)
Hoffie, R.E., Otto, I., Hisano, H. and Kumlehn, J. (2021) Site-Directed Mutagenesis in Barley Using RNA-Guided Cas Endonucleases During Microspore-Derived Generation of Doubled Haploids. In: Doubled Haploid Technology: Volume 1: General Topics, Alliaceae, Cereals (Segui-Simarro, J.M. ed) pp. 199-214. New York, NY: Springer US.
Review (including Japanese)
Hisano, H., Abe, F., Hoffie, R.E. and Kumlehn, J. (2021) Targeted genome modifications in cereal crops. Breeding Science 71, 405-416.