List of Publications


Tominaga, J., Nakahara, Y., Horikawa, D., Tanaka, A., Kamei, Y., Takami, T., Sakamoto, W., Unno, K., Sakamoto, A., and Shimada H. (2018) Overexpression of the protein disulfide isomaerase AtCYO1 in chloroplasts slows dark-induced senescence in Arabidopsis. BMC Plant Biol., 18:80.

Kato, Y. and Sakamoto, W. (2018) FtsH protease in the thylakoid membrane: physiological functions and the regulation of protease activity. Frontiers Plant Sci., 9: 855. doi: 10.3389/fpls.2018.00855.

Nakamura, S., Hidema, J., Sakamoto, W., Ishida, H., and Izumi, M. (2018) Selective elimination of membrane-damaged chloroplasts via microautophagy. Plant Physiol., 177: 1007-1026.

Sakamoto, W. and Takami T. (2018) Chloroplast DNA dynamics: copy number, quality control, and degradation. Plant Cell Physiol., 59: 1120-1127.

Nishimura, K., Matsushita T., Shikanai, T., and Sakamoto, W. (2018) Meeting Report – Taiwan -Japan Plant Biology 2017, Spotlight Issue: From light signals/signaling to photosynthesis and chloroplast development. Plant Cell Physiol., 59: 1099-1103.

Ohnishi, N., Zhang, L., and Sakamoto, W. (2018) VIPP1 involved in chloroplast membrane integrity has GTPase activity in vitro. Plant Physiol. 177: 328-338.

Szymanski, D., Bassham, D., Munnik, T., and Sakamoto, W. (2018) Cellular Dynamics: Cellular systems in the time domain. Plant Physiol. 176: 12-15.

Yamatani, H., Kohzuma, K, Nakano, M., Takami, T., Kato, Y., Hayashi, Y., Monden, Y., Okumoto, Y., Abe, T., Kumamaru, T., Tanaka, A., Sakamoto, W., and Kusaba, M. (2018) Impairment of Lhca4, a subunit of LHCI, causes high accumulation of chlorophyll and the stay-green phenotype in rice. J. Exp. Bot., 69: 1027-1035.


Salesse, C., Sharwood, R., Sakamoto, W., and Stern D. (2017) The rubisco chaperone BSD2 may regulate chloroplast coverage in maize bundle sheath cells. Plant Physiol. 175: 1624-1633.

Kohzuma K, Sato Y, Ito H, Okuzaki A, Watanabe M, Kobayashi H, Nakano M, Yamatani H, Masuda Y, Nagashima Y, Fukuoka H, Yamada T, Kanazawa A, Kitamura K, Tabei Y, Ikeuchi M, Sakamoto W, Tanaka A, Kusaba M.(2017) The Non-Mendelian Green Cotyledon Gene in Soybean Encodes a Small Subunit of Photosystem II.  Plant Physiol. 173:2138-2147.

Nishimura K, Kato Y, Sakamoto W.(2017) Essentials of Proteolytic Machineries in Chloroplasts. Mol Plant. 16:30166-6.


Matsushima, R., Maekawa, M., Kusano, M., Tomita, K., Kondo, H., Nishimura, H., Crofts N., Fujita N. and Sakamoto W. (2016) Amyloplast membrane protein SUBSTANDARD STARCH GRAIN6 controls starch grain size in rice endosperm. Plant Physiology 170: 1445-1459.

Toyosawa, Y., Kawagoe, Y., Matsushima, R., Crofts, N., Ogawa, M., Fukuda, M., Kumamaru, T., Okazaki, Y., Kusano, M., Saito, K., Toyooka, K., Sato, M., Ai, Y., Jane, J.-L., Nakamura, Y. and Fujita, N.(2016) Deficiency of Starch Synthase IIIa and IVb alters starch granule morphology from polyhedral to spherical in rice endosperm. Plant Physiology 170: 1225-1270

坂本亘 光合成の効率向上とスーパーバイオマス.(2016)スーパーバイオマス:植物に学ぶ、植物を活かす(福田裕穂、稲田のりこ編). Pp19-39 慶應大学出版会

Zhang L, Kusaba M, Tanaka A, Sakamoto W.(2016) Protection of Chloroplast Membranes by VIPP1 Rescues Aberrant Seedling Development in Arabidopsis nyc1 Mutant. Front Plant Sci.28;7:533

Zhang L, Kondo H, Kamikubo H, Kataoka M, Sakamoto W.(2016) VIPP1 Has a Disordered C-Terminal Tail Necessary for Protecting Photosynthetic Membranes against Stress.Plant Physiol. 171:1983-95

Nishimura K, Kato Y, Sakamoto W.(2016) Chloroplast Proteases: Updates on Proteolysis within and across Suborganellar Compartments. Plant Physiol. 171:2280-93

Tominaga J, Mizutani H, Horikawa D, Nakahara Y, Takami T, Sakamoto W, Sakamoto A, Shimada H.(2016) Rice CYO1, an ortholog of Arabidopsis thaliana cotyledon chloroplast biogenesis factor AtCYO1, is expressed in leaves and involved in photosynthetic performance. J Plant Physiol. 207:78-83.


Matsushima R, Maekawa M, Sakamoto W. (2015) Geometrical formation of compound starch grains in rice implements voronoi diagram. Plant Cell Physiol. 56:2150-2157. doi: 10.1093/pcp/pcv123.

Oikawa, K., Matsunaga, S., Mano, S., Kondo, K., Yamada, K., Hayashi, M., Kagawa, T., Kadota, A., Sakamoto, W., Higashi, S., Watanabe, M., Mitsui, T., Shigemasa, A., Takanori Iino, T., Hosokawa, Y. and Nishimura, N. (2015) Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis. Nature Plants 1, 15035 ​doi:10.1038/nplants.2015.57

Kato, Y., Ozawa, SI., Takahashi, Y. and Sakamoto W. (2015) D1 fragmentation in photosystem II repair caused by photo-damage of a two-step model. Photosynth Res. 126:409-416. doi: 10.1007/s11120-015-0144-7

Zhang, L., and Sakamoto, W. (2015) Possible function of VIPP1 in maintaining chloroplast membranes. Biochim Biophys Acta.1847:831-7. doi: 10.1016/j.bbabio.2015.02.013.

Kamau, PK., Sano, S., Takami, T., Matsushima, R., Maekawa, M. and Sakamoto, W. (2015) A mutation in GIANT CHLOROPLAST encoding a PARC6 homolog affects spikelet fertility in rice. Plant Cell Physiol. 56:977-91. doi: 10.1093/pcp/pcv024.


Kato, Y. and Sakamoto, W. (2014) Phosphorylation of photosystem II core proteins prevents undesirable cleavage of D1 and contributes to the fine-tuned repair of photosystem II. Plant J. 79:312-321.doi: 10.1111/tpj.12562

Borg, M., Rutley, N., Kagale, S., Hamamura,Y., Gherghinoiu, M., Kumar, S., Sari, U., Esparza-Franco, MA., Sakamoto, W., Rozwadowski, K., Higashiyama, H.andTwell, D. (2014) An EAR-Dependent Regulatory Module Promotes Male Germ Cell Division and Sperm Fertility in Arabidopsis. Plant Cell 26:2098-2113.  tpc.114.124743.

真野昌二・山田(後藤)志野・坂本亘 (2014) 受精とオルガネラ:ミトコンドリア、プラスチド、ペルオキシソーム. 動植物の受精学(澤田均編)化学同人.pp.301-317.

Sakamoto, W. and Takami, T. (2014) Nucleases in higher plants and their possible involvement in DNA degradation during leaf senescence.J.Exp.Bot. 65:3835-3834. doi: 10.1093/jxb/eru091.

Adam, Z. and Sakamoto, W. (2014) Plastid proteases.PlastidBiology (edsTheg, S.andWollman, F.-A.) Advances in Plant Biology (ed. Harada, J.), Springer, 5:359-389.

Shibata, M., Oikawa, K., Yoshimoto, K., Kondo, M., Mano, S., Yamada, K., Hayashi, M., Sakamoto, W., Ohsumi, Y., and Nishimura, M. Plant autophagy is responsible for peroxisomal transition and plays an important role in the maintenance of peroxisomal quality. Autophagy, 10: 936-937.

Wudick, M.M., Luu, D.-T., Tournaire-Roux, C., Sakamoto, W., and Maurel, C. (2014) Vegetative and sperm cell-specificaquaporinsofArabidopsishighlightthevacuolarequipmentofpollenand contribute to plant reproduction. Plant Physiol., 164: 1697-1706.

Matsushima, R., Maekawa, M., Kusano, M., Kondo, H., Fujita, N., Kawagoe, Y., and Sakamoto, W. (2014) Amyloplast-Localized SUBSTANDARD STARCH GRAIN4 Protein Influences the Size of Starch Grains in Rice Endosperm. Plant Physiol. 164: 623-636.

Asai H, Abe N, Matsushima R, Crofts N, Oitome NF, Nakamura Y, Fujita N. (2014) Deficiencies in both starch synthase IIIa and branching enzyme IIb lead to a significant increase in amylose in SSIIa-inactive japonica rice seeds. J Exp Bot. 65:5497-5507.doi: 10.1093/jxb/eru310.

加藤裕介・坂本 亘 (2014) 光合成の光阻害:光化学系IIの損傷と修復の分子メカニズム. 光合成研究と産業応用最前線。NST pp.97-107.

足立太郎・山極寿一・山根裕美・坂本 亘・山科千里・山越 言. (2014)生物学とアフリカの未来. アフリカ研究 85:33-50


Shibata, M., Oikawa, K., Yoshimoto, K., Kondo, M., Mano, S., Yamada, K., Hayashi, M., Sakamoto, W., Ohsumi, Y., and Nishimura. M. (2013) Highly Oxidized Peroxisomes Are Selectively Degraded via Autophagy in Arabidopsis. Plant Cell 25: 4967-4983

Sakamoto, W., Miura, E., and Kato, Y. (2013) A novel link between chloroplast developmentandstressresponselessonedbyleaf-variegated mutant. In Photosynthesis Research for Food, Fuel and Future. 15th International Conference on Photosynthesis. (eds. Kuang, T., Lu, C., and Zhang, L.), pp. 669-673, Springer.

Zhang, L., Kato, Y., Saigo, K., Vothknecht, U.C., and Sakamoto, W. (2013) The lattice-like structure observed by Vipp1-GFP in Arabidopsis chloroplasts. In Photosynthesis Research for Food, Fuel and Future. 15th International Conference on Photosynthesis. (eds. Kuang, T., Lu, C., and Zhang, L.), pp. 394-397, Springer.

加藤裕介・坂本 亘 (2013) 光阻害における光化学系II反応中心タンパク質D1の分解と葉緑体プロテアーゼ. 光合成研究 23(2): 79-85.

Kato, Y.andSakamoto, W. (2013) Plastid protein degradation during leaf development and senescence: Role of protease and chaperones. In Chloroplast Development during Leaf Growth and Senescence, Advances in Photosynthesis and Respiration Vol. 36, pp453-477 (Eds.Biswal, B., Krupinska, K., and Biswal, U.C.), Springer (ISBN 978-94-007-5723-3).

Matsushima R., Yamashita J., Kariyama, S., Enomoto, T., and Sakamoto, W. (2013) A phylogenetic re-evaluationofmorphologicalvariationsofstarchgrainsamong Poaceae species.J.Appl.Glycosci. 60, 37-44.

Nagai M, Ohnishi M, Uehara T, Yamagami M, Miura E, Kamakura M, Kitamura A, Sakaguchi SI, Sakamoto W, Shimmen T, Fukaki H, Reld RJ, Furukawa A, Mimura T. (2013) Ion gradients inxylemexudateandguttationfluidrelatedtotissue ion levels along primary leaves of barley. Plant Cell Environ. 36:1826-1837.

Yamatani H, Sato Y, Masuda Y, Kato Y, Morita R, Fukunaga K, Nagamura Y, Nishimura M, Sakamoto W, Tanaka A, Kusaba M. (2013) NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll-protein complexes during leaf senescence. Plant J. 74: 652-662.

Zhang L, Sakamoto W. (2013) Possible function of VIPP1inthylakoids: Protection but not formation? Plant Signaling Behavior 8: e22860.

Kato Y, Sakamoto W. (2013) Possible compensatory role among chloroplast proteases under excess-light stress condition. Plant Signaling Behavior 8: e23198.


Nakano RT, Matsushima R, Nagano AJ, Fukao Y, Fujiwara M, Kondo M, Nishimura M, Hara-Nishimura I. (2012) ERMO3/MVP1/GOLD36 is involved in a cell type-specific mechanism for maintaining ER morphology in Arabidopsis thaliana PLoS ONE e49103

Zhang L, Kato Y, Otters S, Vothknecht UC, Sakamoto W. (2012) Essential Role of VIPP1 in Chloroplast Envelope Maintenance in Arabidopsis Plant Cell 24: 3695-3707

Kato Y, Sun X, Zhang L, Sakamoto W. (2012) Cooperative D1 Degradation in the Photosystem II Repair Mediated by Chloroplastic Proteases in Arabidopsis. Plant Physiol.  159:1428-1439

MatsushimaR. (2012) Applications of a rapid method to observe starch grains. Bulletin of Applied Glycoscience 2: 147-149

Tang LY, Matsushima R, Sakamoto W. (2012) Mutations defective in ribonucleotide reductase activityinterferewithpollenplastidDNAdegradationmediatedbyDPD1exonuclease. Plant J.70:637-49

Kato Y, Kouso T, Sakamoto W. (2012) Variegated tobacco leaves generated by chloroplast FtsH suppression: implication of FtsH function in the maintenance of thylakoid membranes.
Plant Cell Physiol. 53: 391-404.


Matsushima, R., Tang, L. Y., Zhang L., Yamada, H., Twell, D.andSakamoto, W. (2011)  A conserved, Mg2+-dependentexonucleasedegradesorganelleDNA during Arabidopsis pollen development. Plant Cell 23: 1608-24.

Ozawa, R., Matsushima, R., Maffei, M.E.andTakabayashi, J. (2011) Interaction between Phaseolus plants and two strains of Kanzawa spider mites. Journal of Plant Interactions 6: 125-128.

Tang, L.Y.andSakamoto, W. (2011)  Tissue-specific organelle DNA degradation mediated by DPD1exonuclease. Plant Signaling Behavior 6: 1391-1393.

Sakamoto, W., Miura, E., and Kato, Y. (2011) A novel link between chloroplast development andstressresponselessonedbyleaf-variegated mutant. Proceedings of the 15th International Congress on Photosynthesis (in press)

Zhang, L., Kato, Y., Saigo, K., Vothknecht, U.C., and Sakamoto, W. (2011) The lattice-like structure observed by Vipp1- GFP in Arabidopsis chloroplasts. Proceedings of the 15th International Congress on Photosynthesis (in press)


Zhang, D., Kato, Y., Zhang, L., Fujimoto, M., Tsutsumi, N., Sodmergen, Sakamoto, W. (2010) The FtsH Protease Heterocomplex in Arabidopsis: Dispensability of Type-B Protease Activity for Proper Chloroplast Development. Plant Cell, Nov;22(11):3710-2.

Saika, H., Sakamoto, W., Maekawa, M., Toki, S. (2010) Highly efficient visual selection of transgenic rice plants using green fluorescentproteinoranthocyaninsyntheticgenes. Plant Biotech., in press.

Matsushima, R., Maekawa, M., Fujita, N., Sakamoto, W. (2010) A rapid, direct observation method to isolate mutants with defects in starch grain morphology in rice. Plant Cell Physiol., 51: 728-741.

Piechota, J., Kolodziejczak, M., Juszczak, I., Sakamoto, W., Janska, H. (2010) Identification and characterization of high-molecular-weight complexes by m-AAAproteasesandprohibitinsinmitochondriaof Arabidopsis thaliana. J. Biol. Chem., 285: 12512-12521.

Kato, Y., Sakamoto, W. (2010) New insights into the types and function ofproteasesinplastids. Intl. Rev.Mol. Cell Biol., 161: 185-218.

Miura, E., Kato, Y., Sakamoto, W. (2010) Comparative transcriptome analysis of green/white variegated sectors in Arabidopsis yellow variegated2: responses to oxidative and other stresses in white sectors.J.Exp.Bot., 61: 2433-2445.

Miura E., Kato, Y., and Sakamoto, W. (2010) Reactive oxygen species derived from impaired quality control of photosystem II are irrelevant to plasma-membrane NADPH oxidases. Plant Signaling Behavior. 5: 264-266.


Kato Y, Miura E, Ido K, Ifuku K, Sakamoto W. (2009) The variegatedmutantslackingchloroplasticFtsHsaredefective in D1 degradation and accumulate reactive oxygen species.Plant Physiol. 151(4):1790-801.

Tang LY, Nagata N, Matsushima R, Chen Y, Yoshioka Y, Sakamoto W.(2009) Visualizationofplastidsinpollen grains: involvement of FtsZ1inpollenplastiddivision.Plant Cell Physiol. 50(4):904-8.

Sakamoto W, Uno Y, Zhang Q, Miura E, Kato Y, Sodmergen. (2009) Arresteddifferentiationofproplastidsintochloroplastsin variegated leaves characterized byplastidultrastructureandnucleoidmorphology. Plant Cell Physiol. 50(12):2069-83.

Kato Y, Sakamoto W. (2009) Protein quality control in chloroplasts: a current model of D1 protein degradation in the photosystem II repair cycle.
J Biochem. 146(4):463-9. Review.

Zhang L, Wei Q, Wu W, Cheng Y, Hu G, Hu F, Sun Y, Zhu Y, Sakamoto W, Huang J. (2009) Activation of the heterotrimeric G protein alpha-subunit GPA1suppressestheftsh-mediated inhibition of chloroplast development in Arabidopsis. Plant J. 58(6):1041-53.

Gibala M, Kicia M, Sakamoto W, Gola EM, Kubrakiewicz J, Smakowska E, Janska H. (2009) The lack of mitochondrial AtFtsH4 protease alters Arabidopsis leaf morphology at the late stage of rosette development under short-day photoperiod. Plant J. 59(5):685-99.

Nakano RT, Matsushima R, Ueda H, Tamura K, Shimada T, Li L, Hayashi Y, Kondo M, Nishimura M, Hara-NishimuraI.(2009) GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana. Plant Cell. :3672-85.


MatsushimaR, Hamamura Y, Higashiyama T, Arimura S, Sodmergen, Tsutsumi N, Sakamoto W.(2008) Mitochondrial dynamics inplantmalegametophytevisualizedbyfluorescent live imaging. Plant Cell Physiol. 49(7):1074-83.

Matsushima R, Hu Y, Toyoda K, Sodmergen, Sakamoto W. (2008) The model plant Medicago truncatula exhibits biparentalplastidinheritance.
Plant Cell Physiol. 49(1):81-91.

Yoshida K, Watanabe C, Kato Y, Sakamoto W, Noguchi K. (2008) Influenceofchloroplasticphoto-oxidative stressonmitochondrial alternative oxidase capacityandrespiratory properties: a case study with Arabidopsis yellow variegated 2. Plant Cell Physiol. 49(4):592-603.

Shih CH, Chu H, Tang LK, Sakamoto W, Maekawa M, Chu IK, Wang M, Lo C. (2008) Functional characterization of key structural genes in rice flavonoid biosynthesis.Planta. 228(6):1043-54.

Kmiec-Wisniewska B, Krumpe K, Urantowka A, Sakamoto W, Pratje E, Janska H. (2008) Plant mitochondrial rhomboid, AtRBL12,hasdifferentsubstratespecificityfromitsyeast counterpart. Plant Mol Biol. 68(1-2):159-71.

Arimura S, Fujimoto M, Doniwa Y, Kadoya N, Nakazono M, Sakamoto W, Tsutsumi N. (2008) Arabidopsis ELONGATED MITOCHONDRIA1 is required for localization of DYNAMIN-RELATED PROTEIN3A to mitochondrial fission sites. Plant Cell. 20(6):1555-66.

Sakamoto W, Miyagishima S, Jarvis P (2008) Chloroplast biogenesis: Plastid division, inheritance, regulation, proteinimportandproteome. The Arabidopsis Book,

Sugimoto M, Sakamoto W, Fujitani Y (2008) Localization and expression of serine racemase in Arabidopsis thaliana. Amino Acids, 36(3):587-90

Diaz C, Lemaître T, Christ A, Azzopardi M, Kato Y, Sato F, Morot-Gaudry JF, Le Dily F, Masclaux-Daubresse C. (2008) Nitrogenrecyclingandremobilizationaredifferentiallycontrolledbyleafsenescence and development stage in Arabidopsis under low nitrogen nutrition.Plant Physiol. 147(3):1437-49.

Before 2007